Mahnaz Azarnia; Seyyed Homayoon Sadraee; Franak Najd; Gholamreza Kaka; Mohammad Kamalinejad
Volume 21, Issue 6 , January and February 2015, , Pages 985-992
Abstract
Background: The aim of this study was to evaluate the effects of Elaeagnus Angustifolia extract on mouse embryonic development of Balb/c.
Materials and Methods: Thirty pregnant mice were randomly divided into two groups. Control group consumed drinking water, and the experimental group during pregnancy ...
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Background: The aim of this study was to evaluate the effects of Elaeagnus Angustifolia extract on mouse embryonic development of Balb/c.
Materials and Methods: Thirty pregnant mice were randomly divided into two groups. Control group consumed drinking water, and the experimental group during pregnancy received the aqueous extract at a dose of 500 mg/ kg. Pregnant mice were killed at 18th gestation day and fetal and placental weight and length of the crown-rump of fetuses were measured and recorded. After fixation and tissue processing, liver embryos were taken out and tissue sections were prepared. After staining with hematoxylin-eosin, histomorphometry was investigated. Then obtained results were statistically analyzed.
Results: No apparent abnormality was observed in embryos. The mean of crown-rump length of fetuses in the experimental group was not shown a significantly increased than control group. The mean of fetal weight in the experimental group was significantly decreased than control group. The mean weight of the placenta in the experimental group was significantly reduced compared to control group. In a histomorphomety analysis in the embryo liver of experimental group, with regard to the mean of size percentage of sinusoids and mean number of blood cells significantly increased in experimental group compared to control group. The mean total surface that occupied by hepatocyte and Mean number of hepatocyte nuclei significantly decreased in experimental group compared to control group.
Conclusion: It seems prescribing aqueous extract of Elaeagnus Angustifolia at dose of 500 mg/kg to pregnant mice can cause developmental abnormalities in the fetus and placenta.
Seyyed Homayon Sadraei; MohammadHosein Asadi; Khosro Jadidi; Mostafa Naderi; Gholamreza Kaka; Gholamreza PourHeidari
Volume 17, Issue 4 , January and February 2011, , Pages 229-235
Abstract
Background and Purpose: Eyes are among the most sensitive organs to chemical agents especially Sulfur mustard or Hun Distilled. Therapeutic effects of anti-inflammatory drugs have been shown on the decrease of epithelium of corneal injuries after being exposed to sulfur mustard. The aim of the present ...
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Background and Purpose: Eyes are among the most sensitive organs to chemical agents especially Sulfur mustard or Hun Distilled. Therapeutic effects of anti-inflammatory drugs have been shown on the decrease of epithelium of corneal injuries after being exposed to sulfur mustard. The aim of the present study was to evaluate the protective effects of topically applied Bethametazone - Diclofenac Na in rabbits. Materials and Methods: In this experimental research, thirty six rabbits were used. Animals were randomly divided into six equal groups (6 rabbits in each group) including control, solution and mustard groups and prophylaxis groups included Betamethasone, Diclofenac Na; Betamethasone-Diclofenac Na were applied before being exposed to the sulfur mustard solution. Animals were kept for 2 weeks and the drugs were used 3 times a day for 2 weeks. Slit-lamp examinations were performed under anesthesia before exposure and subsequently at days 1, 2, 5, 7, and 14 after sulfur mustard exposure by ophthalmologists. At the end of the 14th day, specimens of cornea were obtained for ultrastructural evaluation of corneal epithelium. Statistical analysis was performed by one way analysis of variance (ANOVA) with Tukey’s test using SPSS 13 software. Results: No significant differences were found between the control and solvent groups as far as the variables were concerned. Corneal epithelial defect and severe changes in ultrastructure of corneal epithelial surface was found in the sulfur mustard group. Clinically, corneal epithelial defect in prophylactic Diclofenac Na group (4.2±1.32) decreased significantly when compared to the mustard group (68.7±8.42) (P=0.034). Betamethasone-Diclofenac Na group (4.2±1.17) also decreased significantly when compared to the mustard group (68.7±8.42) (P=0.031). The corneal epithelial defect was not seen in prophylactic Betamethasone group. Ultrastructural damage of corneal epithelial surface and their microvillus in Betamethasone group was similar to the control group. Conclusion: Betamethazone is capable of protecting corneal epithelial defect in the eyes of rabbits exposed to sulfur mustard.